LaVision Biotec AsiaPacific

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Ultramicroscope

The LaVision Biotec Ultra-microscope represents an entirely new class of imaging system, created by combining pioneering ideas from the last century with today's technical concepts. It offers exceptional new opportunities for:

  • - Whole brain imaging at cellular resolution
  • - 3D high speed imaging without bleaching
  • - High throughput phenotype screening.

The Ultramicroscope can handle samples of even a centimetre in size, yet with sub-cellular resolution. Based on a technique originally developed for particle analysis in 1903, it has been refined this century for optical sectioning of large biological samples.

LaVision BioTec's Ultramicroscope utilizes a thin sheet of light to excite biological samples while the fluorescence light is detected with a CCD-equipped microscope perpendicularly to the illuminated area. By moving the sample through the sheet and taking pictures at each depth, one generates a 3D image at cellular resolution in whole organs, using fluorescent proteins, fluorescent dyes or autofluorescence!

Principle of Operation

LaVision BioTec's Ultramicroscope utilizes a thin sheet of light to excite biological samples while the fluorescence light is detected with a CCD-equipped microscope perpendicularly to the illuminated area. By moving the sample through the sheet and taking pictures at each depth, one generates a 3D image at cellular resolution in whole organs, using fluorescent proteins, fluorescent dyes or autofluorescence!

Applications

To date juvenile mouse brain, complete mouse embryos as well as whole fruit flies have been successfully analyzed.  A few articles have yet been published based on sheet illumination of biological samples, so the field is open! Whether you want to chart neuronal networks, characterize phenotypes, study vascularization, or something else as yet untested, this is the emerging technology for you!

The Ultramicroscope can be customized to your application! Please ask for: high speed cameras, incubation chambers for in vivo work and high resolution optics.

Specifications

sCMOS camera

Imager 3QE

Detector

Pixel

2560 x 2160

1376 x 1040

Pixel size

6.5 µm x 6.5 µm

6.45 µm x 6.45 µm

Read out noise

< 1.4 e rms @ 33 fps

4 - 6 e rms @ 16 MHz

Excitation Optics

Sheet Illumination (Uni- or Bidirectional)

Thickness

4 - 40 µm

Width

0.1 - 10 mm

Detection

MVX10 with 2XC

Magnification

1.26x - 12.6x

Numerical Aperture

0.5

Working Distance

5.5 mm

Field of View

0.7 - 7 mm

Light source

a) LVBT Laser Module (max. 5 laser diodes)

b) Supercontinuum White Light Laser

Dimensions

540 mm (W) x 700 mm (H) x 550 mm (D)

Weight

47 kg (without Controller and Laser)

Download Brochure: Ultramicroscope Light Sheet Microscopy

Contact:

Phone:

(61) 8 8443 8668

Email:

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